Objective:To study the influence of ethanol on absolute refractory period of sciatic nerve in hoptoad.

探讨乙醇对干绝对不应期的影响。

We used ethanol at different concentrations for the sciatic nerve stem of the unorganized hoptoad and measures its absolute refractory period.

本实验对离体蟾蜍坐骨干进行不同浓度乙醇处理,测其绝对不应期。

Methods The sciatic and brachial plexus nerve of three-day-old SD mice was treated with collagenase type IV and trypsin to remove the desmocyte on the Schwann cell, then explanted in the culture disk.

先用IV型胶原酶和胰蛋白酶消化出生3天的SD鼠臂和坐骨组织块后再将组织块贴壁培养。